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197467

Optimization of bacterial cellulose production using Plackett-Burman and response surface methodology

Article

Last updated: 05 Jan 2025

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Tags

Diagnostics

Abstract

Background: Cellulose is the most widely used natural polymer and can be synthesized by both plants and bacteria. Bacterial cellulose (BC) is a natural biopolymer synthesized by some bacteria, among BC producing bacteria, Komagataeibacter strains have attracted more attention among researchers due to their ability to synthesis BC with high yields. Objective: This study was evaluating the BC production by Komagataeibacter rhaeticus N1 MW32270. Optimizationof BC production by statistical optimization method was studied. Methodology: Plackett-Burman (PB) Design was used for screeningeight tested parameters, then Response surface methodology (RSM) was applied for optimization the significant factors. Results: PB Design revealed that among the eight tested parameters ethanol concentration and incubation time were the most significant factors affecting BC production. RSM was applied for optimization of BC production. The fitted value of ethanol (1.0152%) and incubation time (10 days) were applied; BC production was compared with the predicted value obtained from the optimization plot. According to the achieved results, the BC production by K. rhaeticus N1 MW32270 was 9.2± 1.1g/l in the optimized medium, which agreed well with the predicted values 9.1 g/l, indicating that the model was fitted. Also BC production by K. rhaeticus N1 MW32270in optimized medium was then compared with BC production in the standard Hestrin and Schramm (HS) medium. Our results showed that BC production increased by 115% when compared with BC production in the standard HS medium. Conclusion: Enhancement of BC production by K. rhaeticus N1 MW32270from (4.3± 0.5 g/l) to (9.2± 1.1 g/l) was achieved by using PB and RSM.

DOI

10.21608/ejmm.2021.197467

Keywords

Komagataeibacter rhaeticus, bacterial cellulose (BC), Optimization

Authors

First Name

Nasser

Last Name

Mohammad

MiddleName

H.

Affiliation

Radiation Microbiology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt

Email

nasser.eaea@yahoo.com

City

Cairo

Orcid

-

First Name

Gamal

Last Name

EL-Sherbiny

MiddleName

M.

Affiliation

Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Ali

Last Name

Hammad

MiddleName

A.

Affiliation

Radiation Microbiology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Salwa

Last Name

Abou El- Nour

MiddleName

A.

Affiliation

Radiation Microbiology Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Askar

MiddleName

A.

Affiliation

Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

30

Article Issue

4

Related Issue

27919

Issue Date

2021-10-01

Receive Date

2021-08-25

Publish Date

2021-10-02

Page Start

93

Page End

101

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 4_Pages 93-101.pdf

PDF . 454.4kB

Link

https://ejmm.journals.ekb.eg/article_197467.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=197467

Order

12

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Optimization of bacterial cellulose production using Plackett-Burman and response surface methodology

Details

Type

Article

Created At

23 Jan 2023