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197457

Effect of some medicinal plant extracts on multidrug resistant Helicobacter pylori strains isolated from Sohag and Assiut University Hospitals and some medical laboratories

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Helicobacter pylori is a micro-aerophilic Gram-negative bacteria. The increasing resistance between strains was emerged, resistance to such antimicrobial agents continues to be alarming worldwide. Objectives: to isolation of H. pylori by bacterial culture and evaluate the resistance rate of bacteria to antimicrobials and effect of medicinal plant extracts (A. sativum and C. carvi essential oil, Moringa oleifera, Trigonella foneum, Lawsonia inermis) on H. pylori strains and detection of their effective components.. Methodology: This study was done on stool samples collected from 260 patients attending gastroenterology department at Sohag and Assiut University Hospital, collected during the period from March 2019 to February 2020. The bacterial cultures were done for isolation of the pathogenic bacteria and detection of their antibiotic susceptibility by disc diffusion method and Minimum inhibitory concentration (MICs) determination and treatment of resistant strains with medicinal plant extracts as natural products and detection of their active constituents qualitatively and quantitatively. Results: 87.0% and 59.2% sensitive strains of H. pylori to A. sativum and C. carvi essential oil and 67.4%, 57.1%, 44.9% susceptible strains to T. foneum gracium, M. oleifera, L. inermis were detected. Flavonoids, alkaloids, tannins, phenols as phytochemicals were detected in Moringa oleifera, Trigonella foneum, Lawsonia inermis methanolic extracts causing their antibacterial activity. Disulfide diallyl (30.12%) and limonene (46.39%) and carvone (50.30%) were as bioactive compounds in Allium sativum and Carium carvi essential oil. Conclusion: results, we concluded that the medicinal plant extracts were the most effective agents as antimicrobials so we recommend the use of these extracts as alternatives in the future to treat the resistance problem. 

DOI

10.21608/ejmm.2021.197457

Keywords

Helicobacter pylori, class I carcinogen, Antimicrobial resistance, Medicinal plant extracts

Authors

First Name

Magdy

Last Name

Abu-Gharbia

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Sohag University

Email

-

City

-

Orcid

-

First Name

Michael

Last Name

Agban

MiddleName

N.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University

Email

-

City

-

Orcid

-

First Name

Ayman

Last Name

Hamouda

MiddleName

M.A.

Affiliation

Medicinal and Aromatic Plants Department, Director of Horticulture Research Institute, Agriculture Research Center, Giza

Email

-

City

-

Orcid

-

First Name

Rasha

Last Name

Abdelmasieh

MiddleName

Zaher

Affiliation

Researcher in Medicinal and aromatic plants department, Horticulture research institute, Agriculture research center, Giza

Email

rzaher094@gmail.com

City

Giza

Orcid

-

Volume

30

Article Issue

4

Related Issue

27919

Issue Date

2021-10-01

Receive Date

2021-08-10

Publish Date

2021-10-01

Page Start

65

Page End

73

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 4_Pages 65-73.pdf

PDF . 595.7kB

Link

https://ejmm.journals.ekb.eg/article_197457.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=197457

Order

9

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Effect of some medicinal plant extracts on multidrug resistant Helicobacter pylori strains isolated from Sohag and Assiut University Hospitals and some medical laboratories

Details

Type

Article

Created At

23 Jan 2023