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197454

Screening for Group B Streptococci in Pregnant women by culture and polymerase chain reaction methods

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Diagnostics

Abstract

Background: Group B Streptococcus (GBS) inhabits pregnant women's genitourinary tracts and spreads to the offspring. Objective: This study aimed to screen for GBS colonization in pregnant women, to compare between GBS culture, VITEK® 2 and PCR, and to assess the antimicrobial susceptibility of GBS isolates. Methodology: Vaginal and rectal swabs were collected from 82 pregnant women. GBS was isolated by culture on Strep B Select. Confirmation by VITEK® 2 and PCR for atr and 16S rRNA genes was done. Results: Sixteen vaginal (19.5%) and 22 rectal colonized cases (26.8%) were positive for GBS by culture on Strep B Select agar. GBS was detected in 10 vaginal (12.2%) and 16 rectal colonized cases (19.5%) by VITEK® 2 and PCR for both atr and 16S rRNA genes.  Conclusion: It is suggested that pregnant women should be screened for GBS colonization.  The best precise tests to detect GBS are PCR and VITEK® 2.

DOI

10.21608/ejmm.2021.197454

Keywords

GBS, VITEK® 2, PCR, atr and 16S rRNA genes

Authors

First Name

Aliaa

Last Name

Ghandour

MiddleName

M.A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

aliaaghandour@aun.edu.eg

City

Assiut

Orcid

-

First Name

Ahmed

Last Name

Youssef

MiddleName

A.

Affiliation

Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

Wegdan

Last Name

Mohamad

MiddleName

A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

Rania

Last Name

Bakry

MiddleName

M.

Affiliation

Department of Oncological Clinical Pathology,South Egypt Cancer Institute, Assiut University, Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

Yousra

Last Name

Mammdoh

MiddleName

M

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

Rawhia

Last Name

Fathy

MiddleName

-

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

rawhiafathy@aun.edu.eg

City

-

Orcid

-

Volume

30

Article Issue

4

Related Issue

27919

Issue Date

2021-10-01

Receive Date

2021-06-07

Publish Date

2021-10-01

Page Start

47

Page End

54

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 4_Pages 47-54.pdf

PDF . 442.6kB

Link

https://ejmm.journals.ekb.eg/article_197454.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=197454

Order

7

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Screening for Group B Streptococci in Pregnant women by culture and polymerase chain reaction methods

Details

Type

Article

Created At

23 Jan 2023