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197453

Automated Blood Culture versus Amplification of 16S rRNA Gene Method for Detection of Neonatal Septicemia

Article

Last updated: 28 Dec 2024

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Tags

Diagnostics

Abstract

Background: Accurate and rapid diagnosis of neonatal septicemia is highly warranted because of high associated morbidity and mortality. Blood culture serves as a routine method for diagnosis of bacterial sepsis. However, it sometimes takes ≥ 72 hours for the results, with low sensitivity. Full automated blood culture method is superior to conventional methods in terms of speed and sensitivity. The polymerase chain reaction–based detection of 16S rRNA has reduced the laboratory turnaround time and has good sensitivity. Objectives: The aim of this study was to compare automated blood culture and amplification of 16srRNA gene by PCR in detection of aerobic bacterial infection in blood samples of hospitalized neonates with suspected neonatal sepsis and to determine the most common types of bacteria causing neonatal sepsis. Methodology: Blood samples collected from 40 neonates clinically suspected as neonatal sepsis were subjected to bacterial identification through automated blood culture by BacT/ALERT PF Plus Culture Bottles, and bacterial detection of 16S rRNA gene by PCR. Results: Out of 40 neonatal blood samples with suspected sepsis, 37(92.5%) neonates showed concordance between automated blood culture and PCR; 17(42.5%) showed positive results while 20 (50%) gave negative results by both methods. The remaining 3 cases (7.5%) were positive only by PCR. PCR sensitivity, specificity, positive and negative predictive values were 100%, 86.9%, 85% and 100% respectively. So, accuracy of PCR in relation to automated blood culture is 92.5%. The most common pathogens in cases of early onset neonatal sepsis were Klebsiella pneumoniae (7/15, 46.7%) followed by Streptococcus agalactiae (1/15, 6.6%) while the most common pathogens found in cases of late onset neonatal sepsis were Klebsiella pneumoniae and coagulase negative staphylococci (4/25,16% for each) then Staphylococcus aureus (1/25, 4%). Conclusion: 16S rRNA PCR showed accurate rapid diagnosis with higher sensitivity in diagnosis of neonatal septicemia. Klebsiella peumoniae was the main causative bacteria in early onset neonatal sepsis and late onset neonatal sepsis.

DOI

10.21608/ejmm.2021.197453

Keywords

Neonatal sepsis, 16S rRNA, automated blood culture, EONS, LONS

Authors

First Name

Somaya

Last Name

Desouky

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt

Email

-

City

-

Orcid

-

First Name

Reem

Last Name

Abd ElGlil

MiddleName

-

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt

Email

-

City

-

Orcid

-

First Name

Eman

Last Name

Abd Almonaem

MiddleName

R.

Affiliation

Department of Pediatrics, Faculty of Medicine, Benha University, Egypt

Email

-

City

-

Orcid

-

First Name

Eman

Last Name

Abdelmoneim

MiddleName

Salah

Affiliation

Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt

Email

emansalah205@yahoo.com

City

Benha

Orcid

-

First Name

AL-Shaimaa

Last Name

AL-Tabbakh

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Egypt

Email

-

City

-

Orcid

-

Volume

30

Article Issue

4

Related Issue

27919

Issue Date

2021-10-01

Receive Date

2021-07-13

Publish Date

2021-10-01

Page Start

39

Page End

45

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 4_Pages 39-45.pdf

PDF . 372.4kB

Link

https://ejmm.journals.ekb.eg/article_197453.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=197453

Order

6

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Automated Blood Culture versus Amplification of 16S rRNA Gene Method for Detection of Neonatal Septicemia

Details

Type

Article

Created At

23 Jan 2023