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197273

Enhancement of Taxol Production by Endophytic fungi from Hibiscus and Moringa plant using Gamma irradiation

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Taxol is a commercial anticancer drug, with broad spectrum towards different cancer cell as breast, lung, head and uterine cancers. Taxol producing by endophytes recovered from the medicinal plants. Objective:  This evaluating of Taxol production by endophytes isolated from Moringa and Hibiscus plants. Enhancementof Taxol production by different types of media and Gamma irradiation were studied. The antimicrobial activity of Taxol was observed. Methodology: Fungal isolates were recovered from Moringa and Hibiscus plant. The samples were sterilized with ethanol (70%), wash with sterile water, sterilized tissue by sodium hypochlorite 2.5% then wash with sterile water. The isolates were grown on PDB media, after incubation, the cultures were filtered and characterized by UV-Vis., TLC, HPLC and FTIR. Results:  Twenty seven isolates were separated from Moringa (16 isolates) and Hibiscus plant (11 isolates). These organisms were observed from barks (seven isolates), twigs (eight isolates), leaves (five isolates) and buds (seven isolates) of plants. Purified fungal isolates were Aspergillus, Penicillium, Cladosporium and Fusarium. Between these isolates, the prevalence of genus Aspergillus was detected (70.3%) while Fusarium remark to be (14.8%); also Penicillium was reported (11.1%) and finally Cladosporium (3.7%). From HPLC results the optimum Taxol production was Penicillium sp.5 (54.42µg/L), Aspergillus niger10 (43.95µg/L) and Fusarium sp.8 (26.8µg/L) on potato dextrose agar medium. Conclusion: Improvement of Taxol harvest by Penicillium sp.from (54.42µg/l) to (184.3µg/l) on Dox medium, maximum yield of Taxol was appeared at 1.25 kGy (274.6µg/l). Finally a significant antimicrobial of Taxol towards E. coli 15.0mm and Ent. cloacae 22.0mm.

DOI

10.21608/ejmm.2021.197273

Keywords

Moringa and Hibiscus plant, fungal endophytes, taxol, Gamma Irradiation

Authors

First Name

Sobhy

Last Name

Abdel-Fatah

MiddleName

Sabry

Affiliation

Drug Radiation Research Dep, Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt

Email

sob7y.sabry@yahoo.com

City

Cairo

Orcid

-

First Name

Gamal

Last Name

El-Sherbiny

MiddleName

M.

Affiliation

Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Mahmoud

Last Name

Khalaf

MiddleName

A.

Affiliation

Microbiology Dep, Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

El-Batal

MiddleName

I.

Affiliation

Drug Radiation Research Dep, Biotechnology Division, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

30

Article Issue

4

Related Issue

27919

Issue Date

2021-10-01

Receive Date

2021-07-14

Publish Date

2021-10-01

Page Start

9

Page End

17

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 4_Pages 9-17.pdf

PDF . 623.9kB

Link

https://ejmm.journals.ekb.eg/article_197273.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=197273

Order

2

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Enhancement of Taxol Production by Endophytic fungi from Hibiscus and Moringa plant using Gamma irradiation

Details

Type

Article

Created At

23 Jan 2023