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197271

Characterization of complement activation on the surface of klebsiella pneumoniae

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Host response

Abstract

Background: Life threatening infections caused by the opportunistic pathogen K. pneumoniae are one of the most serious infections that elevate mortality rate in hospitals, worldwide. K. pneumoniae develops several mechanisms for avoiding killing by the complement system for survival in the host. The complement system is the first line of immune defense against different bacteria including K. pneumoniae. Objective: In this study we assessed the role of complement system in killing of both serum resistant and serum sensitive strains of K. pneumoniae. Methodology: Six clinical isolates of K. pneumoniae were collected from urine, sputum and the blood samples from patients in Mansoura Hospitals University. Serum bactericidal effectwas assessed and confirmed by Turbidimetric method, Complement mediated lysis, complements C3b deposition and fH binding were assessed by ELISA. Results: our results demonstrated that complement mediated killing of K. pneumoniae  is driven via the Alternative pathways, it was found that a lower C3b deposition via AP and a higher fH binding to surface of serum resistant K. pneumoniae strains compared with serum sensitive K. pneumoniae Conclusions: This study explains the role of complement system in killing K. pneumoniae and how serum resistant strains escape the immune defense via sequestering complement factor H.

DOI

10.21608/ejmm.2021.197271

Keywords

K. pneumoniae, complement system, Factor H

Authors

First Name

Rana

Last Name

Rabie

MiddleName

-

Affiliation

Gastroenterology Surgical Center, Mansoura University, Mansoura, Egypt

Email

-

City

-

Orcid

-

First Name

Heba

Last Name

Said

MiddleName

S.

Affiliation

Microbiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt

Email

-

City

-

Orcid

-

First Name

Hany

Last Name

Kenawy

MiddleName

I.

Affiliation

Microbiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt

Email

hanykenawy@mans.edu.eg

City

-

Orcid

0000-0002-8548-6649

First Name

Youssif

Last Name

Ali

MiddleName

M.

Affiliation

Microbiology & Immunology Department, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt School of Biological Sciences, Cambridge University, United Kingdom

Email

myima2@cam.ac.uk

City

Mansoura

Orcid

-

Volume

30

Article Issue

4

Related Issue

27919

Issue Date

2021-10-01

Receive Date

2021-06-17

Publish Date

2021-10-01

Page Start

1

Page End

7

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 4_Pages 1-7.pdf

PDF . 538.4kB

Link

https://ejmm.journals.ekb.eg/article_197271.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=197271

Order

1

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Characterization of complement activation on the surface of klebsiella pneumoniae

Details

Type

Article

Created At

23 Jan 2023