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Prevalence of Multidrug-Resistant Tuberculosis Using Phenotypic Drug Susceptibility Testing and GeneXpert MTB/RIF with Characterization of Non-tuberculous Mycobacteria Using MALD

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Multidrug-resistant tuberculosis (MDR-TB) and infections by non-tuberculous mycobacteria (NTM) are diseases of major public health concern. Objective:  The aim of the present work is to study the prevalence and patterns of MDR-TB as well as the characterization of isolated NTM species. Methodology: All samples (1069) were subjected to smear microscopy, culture on Lowenstein-Jensen (LJ) media, and phenotypic drug susceptibility testing (DST) of MTB to isoniazid (INH), rifampin (RF), streptomycin (S), and ethambutol (E). GeneXpert was used for direct detection of MTB and RF resistance. Matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry (MS) was utilized for characterizing isolated NTM species. Results: M.tuberculosis (MTB) was isolated at a rate of 95.3% (1019/1069). MDR-TB was detected at rate of 7.16% with significant patterns for INH + RF + S + E (46.5%) and INR + RF (24.6%) (P-value <0.001). RF resistance was detected at a rate of 27.2% by GeneXpert. Seven NTM species (0.6%) were isolated in culture of which M.porcinum and M.fortuitum had confident identification by MALDI-TOF (score ≥1.8). Conclusion: MDR-TB rate was found to be 7.16% with significant dominance for INH + RF + S + E and INR + RF resistance patterns, while NTM rate was 0.6%.

Authors

First Name

Noha

Last Name

Soliman

MiddleName

Salah

Affiliation

Lecturer of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Egypt.

Email

nsal18@yahoo.com

City

Cairo

Orcid

-

Volume

30

Article Issue

3

Related Issue

26926

Issue Date

2021-07-01

Receive Date

2021-04-16

Publish Date

2021-07-01

Page Start

143

Page End

151

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 3_Pages 143-151.pdf

PDF . 551.4kB

Link

https://ejmm.journals.ekb.eg/article_188928.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=188928

Order

19

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Prevalence of Multidrug-Resistant Tuberculosis Using Phenotypic Drug Susceptibility Testing and GeneXpert MTB/RIF with Characterization of Non-tuberculous Mycobacteria Using MALDI-TOF

Details

Type

Article

Created At

23 Jan 2023