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188076

Phenotypic Methods and Molecular Metallo-β-lactamases Genes Detection in Different Clinical Carbapenems Resistant Acinetobacters Isolates from Tanta University Hospitals

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Acinetobacter species are very common in hospital acquired infections; Carbapenems resistant species become common in hospital and represent a major health problem. Methodology: Our study was carried on 150 patients from ICU; we compared different phenotypic methods and PCR for detection of carbapenems resistance in Acinetobacters. Results: 88.9% of resistant Acinetobacters were detected by Kirby Bauer Disk diffusion method, 54.2%by Modified Hodge test, 70.8 % by EDTA disc synergy, 85.4% by Amp C disk test, 33.3% by PCR bla-IMP and 12.5% by PCR bla-VIM. Conclusion: We recommended for detection of carbapenems resistance in Acinetobacters the phenotypic methods as they are rapid, accurate methods and less costly than PCR.   Note: bla-IMP gene (NCBI GenBank Nucleotide accession #  LC103138.1); bla-VIM gene  (NCBI GenBank Nucleotide accession # MT185944.1   GenBank accession number S71932

Keywords

Acinetobacters, Carbapenems resistance genes, Metallo Beta Lactamases

Authors

First Name

Kareman

Last Name

Eshra

MiddleName

Ahmed

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta University

Email

kareman.eshra@med.tanta.edu.eg

City

Tanta

Orcid

-

Volume

30

Article Issue

3

Related Issue

26926

Issue Date

2021-07-01

Receive Date

2021-03-08

Publish Date

2021-07-11

Page Start

89

Page End

95

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 3_Pages 89-95.pdf

PDF . 416.9kB

Link

https://ejmm.journals.ekb.eg/article_188076.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=188076

Order

12

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Phenotypic Methods and Molecular Metallo-β-lactamases Genes Detection in Different Clinical Carbapenems Resistant Acinetobacters Isolates from Tanta University Hospitals

Details

Type

Article

Created At

23 Jan 2023