Background: Heteroresistance described infections with bacterial strains which causes infections with different levels of resistance to an antibiotic and differs with age and gender. Objectives: This work determined the variations in the heteroresistance pattern for the age, gender and site of infection and the statistical analysis was done using (SPSS version 20.0) for test of significance. Methodology: Out of two hundred and fifty clinical specimens isolated from different sites from Inpatients admitted to Kasr Al-Aini hospital and Al-Demerdash hospital from different genders with different ages in the period from February 2016 to December 2017, Egypt, one hundred and forty five specimens revealed P.aeruginosa after passing several conventional microbiological methods, the antimicrobial susceptibility testing and the screening of nht4heteroresistant specimens were performed. Results: The obtained results showed that the hetesroresistance was very high in the urine specimens and very high in males more than females. Conclusion: From the statistical analysis we recommend the use of imipenem, levofloxacin and ciprofloxacin in the treatment of heteroresistant P.aeruginosa, since they were the highly significant, effective antibiotics.