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188073

The Identification of Microbial Pathogens Using Matrix Assisted Laser Desorption Ionization Time of Flight in Medical Intensive Care Unit Beni-Suef University Hospital

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Clinical microbiology

Abstract

Background: Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) is a novel technique for identification of microbes. This new method led to a new era in microbial identification because of its rapid, accurate, valid, simple and relatively decreased cost. Objectives: The aim of this study was identification of predominant pathogens by MALDI-TOF technique. Methodology: Pathogens were identified by both conventional methods and MALDI-TOF. Results: From July till December 2018, predominant pathogens were Klebsiella pneumoniae (21%), Pseudomonas aeroguinosa and Candida each constitutes (17%), E-coli (10%), Staph. aureus (9%), Acinetobacter (9%). Identification of isolates (from September to December 2018) by MALDI-TOF revealed a total agreement of (94.1%) with conventional method at genus level, (88.2%) at level of species. Kappa agreement revealed almost perfect correlation between both techniques. Conclusion: The MALDI-TOF results might suggest that its' usage may be dependable for microbiological identification.

Keywords

MALDI -TOF, Microbiological Identification, Conventional Methods

Authors

First Name

Marwa

Last Name

Mohamed

MiddleName

Nabil

Affiliation

General Directorate of Infection Control, Ministry of Health and Population

Email

marwanabil9304@yahoo.com

City

Beni-Suef

Orcid

-

Volume

30

Article Issue

3

Related Issue

26926

Issue Date

2021-07-01

Receive Date

2021-08-07

Publish Date

2021-07-01

Page Start

65

Page End

69

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 3_Pages 65-69.pdf

PDF . 349.8kB

Link

https://ejmm.journals.ekb.eg/article_188073.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=188073

Order

9

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

The Identification of Microbial Pathogens Using Matrix Assisted Laser Desorption Ionization Time of Flight in Medical Intensive Care Unit Beni-Suef University Hospital

Details

Type

Article

Created At

23 Jan 2023