Beta
ManageSearchChatDiscover
188059

SFO-1 and DHA-1 genes Detection in β-lactamases-producing Klebsiella pneumoniae Causing Blood Stream Infections Acquired in Intensive Care Units in Mansoura University Hospitals

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial agents

Abstract

Objectives: The study aimed to detect SFO-1 and DHA-1 genes and to differentiate AmpC with overlapped ESBL isolates. Also, it identified the risk factors aiding in emergence the ESBL producing K.pneumoniae causing blood stream infections in ICUs of Mansoura University Hospitals (MUHs). Methodology: This is a prospective study that enrolled 520 blood samples. Double disk synergy test (DDST) and Modified three-dimensional test were performed. Genotypic detection of SFO-1, Class A ESBL, and DHA-1, AmpC β-lactamase was done. Results: 520 septicemic patients were enrolled with significant correlation for adults' acquisition of infection. The main bacteria isolates causing nosocomial infection in septicemic patients admitted to ICU were S. aureus, E. coli and K. pneumoniae. A significant difference in distribution of ESBL and AmpC β-lactamases was detected with significant association between impenem resistance and prevalence of ESBL in those patients. There was a low-occurrence of ESBL SFO-1 and DHA-1 detected in clinical samples. Surgical intervention and CVC were the significant risk factors for presence of ESBL but previous antibiotics and hospital stay were non-significant effectors for presence of ESBL. Conclusion: Though SFO-1 is a low-prevalence ESBL, it has been taken by a plasmid with many other multiple resistance determinants including many related genes, and go together with by a large DHA-1- plasmid. 

Authors

First Name

Ghada

Last Name

Barakat

MiddleName

-

Affiliation

Department of Medical Microbiology & Immunology, Faculty of Medicine, Mansoura University, Egypt

Email

barakat.ghada@yahoo.com

City

Mansoura

Orcid

-

Volume

30

Article Issue

3

Related Issue

26926

Issue Date

2021-07-01

Receive Date

2021-03-25

Publish Date

2021-07-11

Page Start

43

Page End

51

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 3_Pages 43-51.pdf

PDF . 604.5kB

Link

https://ejmm.journals.ekb.eg/article_188059.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=188059

Order

6

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

SFO-1 and DHA-1 genes Detection in β-lactamases-producing Klebsiella pneumoniae Causing Blood Stream Infections Acquired in Intensive Care Units in Mansoura University Hospitals

Details

Type

Article

Created At

23 Jan 2023