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187001

The impact of the nucleotide-binding oligomerization domain 1 (NOD 1) gene polymorphism on Helicobacter Pylori induced chronic gastritis in hepatic patients

Article

Last updated: 23 Jan 2023

Subjects

-

Tags

Genetics

Abstract

Background:Chronic infection with Helicobacter pylori (H.pylori ) causes atrophic and even  gastric metaplastic changes, and it has a well-known link to peptic ulceration. Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) is a protein receptor that is presented by the NOD1 gene. It distinguishes H.pylori bacterial molecules and enhances an immune response Objectives:  to describe the relation between the NOD1 gene (rs2075820) polymorphism and H.pylori infection in hepatic and non hepatic patients with chronic gastritis, study its impact on the degree of chronic gastritis in H.pylori positive individuals, and to examine the effect of H. pylori on clinical, endoscopic and histopathological findings and child paugh scoring in hepatic patients. Methodology: Gastric tissue samples were taken from selected 200 patients with chronic gastritis, either hepatic or non hepatic. Rapid urease test and pathological findings classified them into H.pylori infected and non infected patients. Genotyping of NOD 1 was studied using polymerase chain reaction /restriction fragment length polymorphism (PCR–RFLP) method. Results: A significant higher frequency of AA genotype, and the A allele of NOD1 gene in H.pylori +ve patients, either hepatic; (58%)-(73%) or non hepatic;(62%)-(78%) as compared to H.pylori –ve  patients,(P <0.001). A highly significant relation between NOD1 genotypes  and endoscopic findings, where most of H.pylori infected patients with AAgenotype had more peptic ulcer, antral erosion, gastric prolapse, esophageal varices and esophageal hiatus hernia compared to patients with GA and GG genotypes, (P<0.001). No significant impact of H.pylori on signs of liver affection and child paugh scoring in hepatic patients.  Conclusions: In NOD1 gene polymorphism, AA genotype and A allele have significantly implicated in H.pylori  infection susceptibility  and progression. While GG genotype and G allele have a protective effect against H.pylori  infection.

Keywords

H.Pylori, Chronic gastritis, hepatic patients, NOD 1 gene polymorphism, Gastric outcomes (endoscopic &pathological findings

Authors

First Name

Eman

Last Name

Hassan

MiddleName

-

Affiliation

Assistant Lecturer of Clinical Microbiology and Immunology, National Liver Institute, Menoufia University Menoufia, Egypt

Email

dr.emanhelemy2024@gmail.com

City

Menoufia

Orcid

-

Volume

30

Article Issue

3

Related Issue

26926

Issue Date

2021-07-01

Receive Date

2021-03-09

Publish Date

2021-07-11

Page Start

9

Page End

19

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_187001.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=187001

Order

2

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

-

Details

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Article

Created At

23 Jan 2023