Asparagus adscendens Roxb. (Asparagaceae) also known as Shatavar Bhed in Ayurvedic medicine, is used for the treatment of female disorders. Traditionally, it is also used in diarrhea, dysentery, leucorrhoea and general debility. Saponins and stigmasterol glycosides were reported from A. adscendens. It is distributed in the Western Himalayas regions of India. Twenty five germplasms of A. adscendens were collected from Himachal Pradesh and Uttarakhand in the year 2008 and were multiplied in the experimental field of ICAR-DMAPR, Anand. Saponin content expressed as shatavarin IV was estimated in methanolic extract of harvested roots of A. adscendens using HPLC- ELSD. The wide range of variation (0.45 – 5.29%) were recorded in the 25 accessions of A. adscendens. DAA5 (5.29%), DAA25 (5.11%), DAA26 (4.74%), DAA2 (4.35%) and DAA28 (3.70%) were identified as accessions with high saponin content which could be exploited for their potentialities. Germplsams with high saponin content could be used for further multiplication in research progamme for development of agrotechniques for ensuring raw material of standardized content and quality.