The new synthesized bioactive Ni(II) isatin-hydrazone complexes have been investigated in presence of Britton– Robinson buffer “universal buffer" as supporting electrolyte, using at glassy carbon electrode. The CV of these complexes is characterized by one cathodic peak in the forward direction at -0.535 V and one anodic peak in the reverse direction at 0.811 V. The two waves considered as two independently irreversible cyclic voltammetric peaks. The CT-DNA binding property of the Ni(II) isatin-hydrazone complexes has also been characterized using cyclic voltammetry under physiological conditions (pH 7.4 and 25 ± 0.2 0C). As a result of the interaction of the subject complexes with CT-DNA, a considerable decrease in the peak currents and a significant shift in the peak potentials were observed. These changes in the cyclic volmmetric characteristics of Ni(II) isatin-hydrszone complexes indicated that these complexes bind to CT-DNA by intercalative mode with intrinsic binding constants in the range of 10-4 M-1.