Cultural heritage materials degrades over time, however conservation slows 
down the speed of its deterioration. Microbiological contamination with bacteria can 
pose a big destroy to old manuscripts and mummy or hazard to those working in 
archives or library.Most of the biological damage is started in poor environmental 
conditions for storage and display.The gas chromatography/mass spectrometry (GC/MS) 
analysis of the oils revealed the main constituents of the essential oils Eucalyptus 
camaldulensis, spathulenol (20.84%), eucalyptol (12.01%), sabinene (9.73%), αphellandrene (8.18%), crypton (7.69%), terpinen-4-ol (3.69%), phellandral (3.54%) and 
D-limonene (2.28%), and Citrussinensis, D-limonene (73.4%) and -terpinene (22.6%).
This work aimed to access the presence of microorganisms and their effect on the old 
manuscripts, mummy deterioration; it also studies their treatment methods, such as 
fumigation natural oils.The causes of the various efficacy of the treatments are 
observed, also because the potential risks of recolonization by viable cells left behind 
after treatment. The results further showed that the test oils were able to inhibit the 
mycelia growth by usingthe fumigation method.The potent properties of E. 
camaldulensis, the possibility of using it as eco-friendly, safe, and cost-effective 
antibacterials for bacterial that could cause discoloration of the archaeological organic 
materials.