The functionality of biomaterials is providing some advantages which enable it for 
targeted application these advantages are limited by the available techniques which is 
a topic of research to continue in the establishment of spectroscopic tools for 
investigation to follow up the changes in this critical and vital field of research.
Biomaterials and biological Molecules investigation needs certain care according to 
their sensitive role in one hand and low detection limits on the other hand. Many 
spectroscopic tools are already introduced including Raman technique, fluorescence 
as well as techniques based on single molecule detection.
The introduced techniques depending on conventional spectroscopic methods also it 
depends on imaging. 
Biomaterials are achieved after blending and/or introduction of matrix include part or 
full metal oxides nano materials in order to facilitate the process of functionalization 
based on the desired applications.