Background: As surgeons embrace the concept of increasingly less invasive surgery, techniques using only a single small incision have begun to gain traction. Single-port access cholecystectomy is a new laparoscopic procedure using only one transumbilical placed port. We report one of the initial clinical experiences at Ain Shams University Hospitals with this new technique. Our study aimed to assess whether these devices allow safe and reliable access for laparoscopic cholecystectomy (LC). Methods: From December 2009 to March 2010, single port laparoscopic cholecystectomy was performed on 10 consecutive patients with mean age ±S.D. 31.0± 11.879 years (range, 18- 50) years. The patients were 8 females and 2 males with mean weight ±S.D. 66.700± 10.252kg (range, 50-80) kg. Diagnoses for cholecystectomy were: chronic calcular cholecystitis (n=7), gallbladder polyp (n=1) and chronic non calcular cholecystitis (n=2) with exclusion of patients with acute cholecystitis, history of jaundice, biliary pancreatitis and prior abdominal surgery. These restrictions were to facilitate the procedure since we are still in the beginning of the learning curve. All procedures were done using the SILSTM port (Covidien, Inc., Norwalk, CT, USA). In our study, the operative time, reasons for conversion to standard four port laparoscopic surgery, per operative and postoperative complications, as well as hospital stay were studied. Results:  Single port laparoscopic cholecystectomy was feasible in 8 cases (80%) 7 females and 1 male. Two cases (20%) were converted to four port laparoscopic surgery. No per operative or post operative complications were recorded. The operative time was longer than in common laparoscopic cholecystectomy with mean time ±S.D. 125.0±37.796 (range, 90-180) minutes. The mean hospital stay ±SD. was 29.66±4.4 (range, 24-36) hours. Conclusion: The results from this study show that single port laparoscopic cholecystectomy seems to be safe and feasible when performed by experienced laparoscopic surgeon.