A total of 200 unsexed 1-day old broiler chickens (Masr poultry) were used in this study in El-Dakhla City in the New Valley Governorate. They were randomly allocated into two groups of 100 birds in each. Groups were classified according to water source, (G1) received superficial well water (S) and (G2) received filtered water (F), that have been physically and chemically analyzed. The two groups received balanced formulated diet free from any additives and vaccinated with AI (H9) and ND vaccines. Weekly, five chicks were randomly collected and weighted (FI, BWG and FCR were estimated), At (1, 7, 15, 25, 35 days) 5 serum samples were examined for the antibody titers of AI (H9) and ND vaccines using HI test. The results of physicochemical water analyses recorded higher numerical values in superficial well group than filtered group which exceeded the permissible limits. There are significant differences among groups at (p>0.05) for most of the performance and immune parameters. (G2) showed a significant higher response for body weight (BWT), BWT gain (BWG), feed conversion ratio and antibody response to ND and AI (H9) virus vaccine. Our study showed that concentration of heavy metals residues in different tissues of broilers received Superficial well as (Fe, Mn, Pb and CD) recorded higher result as compared with filter group. In conclusion, In El Dakhla City the superficial well water affect negatively on performance and immune parameters of broiler chickens.