Background: Root canal treatment is considered one of the most important and challenging procedures in the field of dentistry and the modern daily dental practice. Procedural Errors during root canal treatment occurs often due to lack of knowledge of the root canal system. One of these common challenging errors is tooth or root perforation. Sealing of such defects is a must to eliminate and inhibit any bacterial infection. Objectives: To assess the sealing ability by dye penetration method and dislodgement resistance of three different perforation repair materials namely MTA, nano-hydroxyapatite and hydroxyapatite. Materials and Methods: Sixty extracted human molars were selected for the study. All teeth had a standard endodontic access cavity preparation to expose the pulp chamber. Using a carbide rose head bur size # 2, a furcation perforation was created. All Teeth were divided into three experimental groups (n=20) according to type of perforation repair material used. Repaired perforation sites were evaluated for sealing ability and push-out bond strength of the repair materials by using Stereomicroscope and universal testing machine respectively. Results: MTA showed the least amount of dye penetration and its resistance to dislodgment was the best Conclusion: MTA can be considered the material of choice for the treatment of furcation perforation as compared to Hydroxyapatite and Nanohydroxyapatite.