Background:Measuring and comparing shear bond strength after cementation by resin cement when using desensitizing agents versus diode laser in dentin desensitization. Objectives: To evaluate the difference between desensitizing agents and diode laser on shear bond strength between dentin and resin cement. Materials and methods: 2 mm standardized dentin samples were obtained from thirty molars to form sixty samples that were divided randomly into five groups (n=12). Group C (no surface treatment), Group GL (Gluma desensitizing agent was applied on dentin, Group BF (Bifluoride desensitizing agent was applied on dentin), Group DL2 (Diode laser 2W was irradiated on dentin) and Group DL3 (Diode laser 3W was irradiated on dentin). All samples were cemented by resin cement then tested for shear bond strength using a universal testing machine. Data obtained was analyzed using one-way (ANOVA) (P ≤ 0.05) then Tukey post-hoc test. Representative samples from each group were analyzed using SEM. Results: There was significant difference between all groups but there was no significant difference between group (C, GL, DL2 and DL3) (10.87 MPa, 12.88 MPa, 9.81 MPa and 10.64 MPa) respectively. Significant difference was found between group (GL and BF) (12.88 MPa and 7.33 MPa) respectively. Lowest resin cement infiltration was observed under SEM in group (BF) having lowest shear bond strength. Conclusion: Bifluoride treatment had least shear bond strength and least resin cement infiltration, better not to be used in dentin desensitization before cementation.