Different applications used nano materials such as Nano-Oxides or Nano-Salts i.e.  silver nanoparticles (Ag NPs.) or zinc oxide Nano-particles (ZnO NPs.) to acquire antibacterial activity for cotton fabrics. In the present study chitosan treated cotton fabrics followed by nano treatment via Ag NPs. or ZnO NPs., or mixture of both nano-materials had been subjected to UV irradiation using medium pressure mercury lamp with a light intensity on the fabric of about 15mW/cm2 to produce an enhancement in the anti-bacterial activity against Gram +ve and Gram –ve bacteria i.e. St. coccus and E. coli. The produced yield was assured with FT-IR and scanning electron microscope. The best result for Gram negative bacteria (Escherichia coli) obtained by using mixture of (Ag NPS (100ppb) + ZnO NPS (8% o.w.s) + UV) as well as for Gram positive bacteria (Staphylococcus Aureus) the best result obtained by using mixture of (Ag NPS (100ppb) + ZnO NPS (8% o.w.s) + UV).