Microelements and plant growth regulators are essential to sustain the crop  production of traditional and organic farming systems. Thus, a 2–year field experiment was conducted the Experimental Farm, Faculty of Agriculture, Fayoum University at "Demo" district, Fayoum Governorate, Egypt during the two successive seasons of 2018-2019 and 2019-2020 The study aimed to examine the effects of zinc sulfate, cytokinin rates and yeast extract concentrations and their interactions on yield and yield attributes of wheat plants. Along the two seasons, yeast extract concentrations had statistically significant effect on plant height, spikes number per plant, 1000 grain weight, grain yield, straw yield and crud protein percentage. Wheat plants treated with 0.50 ml/liter cytokinin rate surpassed to untreated plants on plant height, spikes number per plant, 1000 grain weight, grain yield and straw yield  in two seasons (2018-2019) and (2019-2020). A significant effect in 1000 grain weight, grain yield, straw yield and crud protein percentage were obtained by spraying wheat plants with zinc sulfate concentrations in both seasons. Interaction between zinc sulfate (1 g/liter) x cytokinin rate (0.50 ml/liter) and yeast extract concentration (5 or 10 g/liter) showed that producing the highest values of grain yield (ton/fed) and straw yield (ton/fed). Also, the data reflect appositive result of this chemical substance on maximization wheat productivity under these experimental conditions.