The present experiment was carried out at the apiary of the Bee Research Dept., Plant Protection Res. Inst., Agric. Res. Cent., Ministry Agric., Dokki, Giza, Egypt in August 2018 to study the effect of venom collection on hygienic and hoarding behavior in two honeybee races; i.e., carniolan, A.m. carnica and Italian, A.m. ligustica. Results indicated that hygienic behavior significantly increased (P < 0.05) after bee venom collection than before collection in both races and there were insignificant differences between the two races in all tested intervals. While hoarding behavior significantly decreased after bee venom collection than before collection in both races and there were insignificant differences between the two races in all tested intervals.