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197844

IN VITRO EVALUATION OF THE ANTIOXIDANT EFFECTS OF SOME BOTANICAL ETHANOLIC EXTRACTS AND ISOLATION OF THE ANTIOXIDANT CONSTITUENT(S) FROM RUTA CHALEPENSIS LEAVES

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Last updated: 28 Dec 2024

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Abstract

The antioxidant effects of aqueous ethanolic extracts (80 %)from
the leaves of four plants i.e. Ruta chalepensis, Gomphocarpus sinaicus,
Segertia thea and Acacia holoseriaca were evaluated in vitro by
determining their effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH)
decoloration, inhibition of DNA-sugar damage and inhibition of lipid
peroxidation. Among the four extracts tested, Ruta chalepensis and
Gomphocarpus sinaicus extracts showed the strongest antioxidant
activity. The ethanolic extract of Ruta chalepensis leaves was subjected
to isolation and structural characterization of the active constituent(s) of
this plant. Two active flavonoid compounds were isolated by using a
combination of chromatographed methods (TLC and column
chromatography). Their chemical structures were characterized by
spectroscopic methods (UV, 1H and 13C-NMR) as Quercetin 3-O-
[rhamnosyl (1-6) glucoside] (I) and Isorhamnetin 3-O-glucoside-7-Orhamnoside (II).

DOI

10.21608/fjard.2005.197844

Keywords

Natural antioxidants, Ruta chalepensis, Lipid peroxidation, Flavonoids, DPPH

Authors

First Name

Mostafa M.

Last Name

Farag

MiddleName

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Affiliation

Biochemistry Department, Faculty of Agric., Cairo Univ., Giza Egypt.

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First Name

Ahmed M.

Last Name

Emam

MiddleName

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Affiliation

Biochemistry Department, Faculty of Agric. Fayoum Branch, Cairo Univ., Egypt.

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Orcid

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First Name

Mamdouh A.

Last Name

Mohamed

MiddleName

-

Affiliation

Biochemistry Department, Faculty of Agric. Fayoum Branch, Cairo Univ., Egypt.

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Volume

19

Article Issue

2

Related Issue

27974

Issue Date

2005-07-01

Receive Date

2021-10-03

Publish Date

2005-07-01

Page Start

178

Page End

190

Print ISSN

1110-7790

Online ISSN

2805-2528

Article File

FJARD_Volume 19_Issue 2_Pages 178-190.pdf

PDF . 810.7kB

Link

https://fjard.journals.ekb.eg/article_197844.html

Detail API

https://fjard.journals.ekb.eg/service?article_code=197844

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16

Type

Research articles.

Type Code

1,920

Publication Type

Journal

Publication Title

Fayoum Journal of Agricultural Research and Development

Publication Link

https://fjard.journals.ekb.eg/

MainTitle

IN VITRO EVALUATION OF THE ANTIOXIDANT EFFECTS OF SOME BOTANICAL ETHANOLIC EXTRACTS AND ISOLATION OF THE ANTIOXIDANT CONSTITUENT(S) FROM RUTA CHALEPENSIS LEAVES

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Article

Created At

23 Jan 2023