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197566

BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF AN EGYPTIAN ISOLATE OF SOYBEAN MOSAIC VIRUS IN EGYPT.

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Last updated: 05 Jan 2025

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Abstract

Soybean mosaic Potyviridae (SbMV) was isolated from naturally
infected soybean plants cvs. Giza 111 and Giza 35 grown at Giza
Research Station Farm, Agricultural Research Centre, Giza. The
inoculated indicator plants gave severe mosaic on soybean cv.Giza 111,
mosaic and malformation on cv Giza 35, mild mosaic on cv. Giza 22,
yellow mild mosaic on cv. Giza 83, vein clearing and mild mosaic on
Phaseolus vulgaris cvs Contender. Chlorotic local lesion on
Chenopodium quinoa. The virus was transmitted in non-persistent
manner by aphids. Electron Microscopy showed aggregates of flexuous
virus particles, degenerated mitochondria and inclusions appeared as
pinwheel in cytoplasm of infected soybean leaves. Dot blot
immunoassay (DBIA) and indirect enzyme linked immunosorbent assays
(ELISA) were used as a serological method for detection and
confirmation of SbMV isolate in infected tissues.A reverse transcription–
polymerase chain reaction (RT-PCR) method was used to detect of local
isolate of SbMV in infected plants using specific oligonucleotides
primer. A major RT-PCR fragment (~340 bp) from the coat protein gene
of the SbMV genome was detected.

DOI

10.21608/fjard.2006.197566

Keywords

SbMV, aphid transmission, DIBA, electron microscopy, RT-PCR

Authors

First Name

Mohga M.

Last Name

El-Tahlawey

MiddleName

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Affiliation

Plant Virology Research Department, Plant Pathology Research Institute, A.R. C., Giza-Egypt.

Email

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City

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Orcid

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First Name

M. A.

Last Name

Amer

MiddleName

-

Affiliation

Plant Virology Research Department, Plant Pathology Research Institute, A.R. C., Giza-Egypt.

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Mandour

MiddleName

-

Affiliation

Plant Virology Research Department, Plant Pathology Research Institute, A.R. C., Giza-Egypt.

Email

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City

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Orcid

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Volume

20

Article Issue

1

Related Issue

27951

Issue Date

2006-01-01

Receive Date

2021-10-02

Publish Date

2006-01-01

Page Start

40

Page End

50

Print ISSN

1110-7790

Online ISSN

2805-2528

Article File

FJARD_Volume 20_Issue 1_Pages 40-50.pdf

PDF . 819.7kB

Link

https://fjard.journals.ekb.eg/article_197566.html

Detail API

https://fjard.journals.ekb.eg/service?article_code=197566

Order

4

Type

Research articles.

Type Code

1,920

Publication Type

Journal

Publication Title

Fayoum Journal of Agricultural Research and Development

Publication Link

https://fjard.journals.ekb.eg/

MainTitle

BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF AN EGYPTIAN ISOLATE OF SOYBEAN MOSAIC VIRUS IN EGYPT.

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Article

Created At

23 Jan 2023