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190559

A SIMPLE, RAPID, EFFICIENT AND LOW COST METHOD FOR MINIPREP DNA FROM DIFFERENT SOURCES

Article

Last updated: 05 Jan 2025

Subjects

-

Tags

Agricultural Economics and Management Sciences

Abstract

DNA extraction and purification are routine processes in most
plant genetic transformation laboratories. Although there are different
commercial kits that allow accurate DNA purification, the total cost of
buying multiple sets of these kits can be spectacular. We use spin
column and laboratory solutions to develop a simple method of DNA
purification that can meet different research needs. This method is used
to extract DNA from the leaves of Brassica and genetically modified
plants and also bacteria; extract plasmid DNA from
E. coli or
Agrobacterium tumefaciens; purify the DNA fragments of PCR
products and the resulting fragments of digestion with restriction
endonuclease. DNA concentration of optical density (OD) value was
calculated at 260 nm wavelength and OD260 / OD280 ratios were used
to determine DNA quality. The quantity and quality of DNA obtained
by this method was similar to that of isolated DNA using commercial
Kits. In comparison, it has been shown that this method allows
obtaining DNA from different sources with similar quantity and purity
and low costs.


DOI

10.21608/fjard.2019.190559

Keywords

DNA purification, Brassica plant, Escherichia coli, Agrobacterium tumefaciens, plasmid DNA, quantity and quality of DNA

Authors

First Name

Zaki

Last Name

A. El Fiky

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, Fayoum University, Fayoum

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

A.M. Yassein

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, Fayoum University, Fayoum

Email

-

City

-

Orcid

-

First Name

Nada

Last Name

F. Hemeda

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, Fayoum University, Fayoum

Email

-

City

-

Orcid

-

First Name

Gehad

Last Name

R. Eid

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, Fayoum University, Fayoum

Email

-

City

-

Orcid

-

Volume

33

Article Issue

2

Related Issue

27239

Issue Date

2019-07-01

Receive Date

2021-08-22

Publish Date

2019-07-01

Page Start

12

Page End

23

Print ISSN

1110-7790

Online ISSN

2805-2528

Link

https://fjard.journals.ekb.eg/article_190559.html

Detail API

https://fjard.journals.ekb.eg/service?article_code=190559

Order

2

Type

Research articles.

Type Code

1,920

Publication Type

Journal

Publication Title

Fayoum Journal of Agricultural Research and Development

Publication Link

https://fjard.journals.ekb.eg/

MainTitle

A SIMPLE, RAPID, EFFICIENT AND LOW COST METHOD FOR MINIPREP DNA FROM DIFFERENT SOURCES

Details

Type

Article

Created At

23 Jan 2023