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256166

CALLOGENESIS AND PLANT REGENERATION VIA IN VITRO CULTURE OF STEVIA REBAUDIANA EXPLANTS

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Last updated: 28 Dec 2024

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Abstract

Tissue culture technique became one of the most important tools in plant breeding. Callus is important for rapid mass multiplication, generation of variability, cell suspension culture, preservation of cell line culture and production of secondary metabolites. Therefore, this study was carried out on stevia plant for establishing efficient methods of formation and regeneration of callus. Three different explants (shoot tip, leaves and nodal segments) and Murashige and Skoog medium (MS) with different concentrations and combinations of growth regulators (PGRs) were used to determine which explant is the most suitable for callus induction and regeneration in the presence of different PGRs. All studied media induced callus for all explants, but MSc4 (MS+1.0 mgl-1 2,4-D +0.75 mgl-1 NAA) gave the highest values of callus fresh weight. Only, calli obtained from Msc4 callus induction medium gave the best response to regenerate a sufficient number of shoots. Half strength MS medium with 1 mgl-1IBA was found to be the optimum medium for root formation. It gave a good root formation (88.67%), highest roots number/shoot (6.24), and highest root length (2.90cm).

DOI

10.12816/ejpb.2019.256166

Keywords

Stevia rebaudiana, Explants, Plant growth regulators, callus formation, Plant regeneration

Volume

23

Article Issue

1

Related Issue

36340

Issue Date

2019-08-01

Receive Date

2022-08-25

Publish Date

2019-08-01

Page Start

65

Page End

76

Print ISSN

1110-7863

Online ISSN

2735-3885

Article File

EJPB_Volume 23_Issue 1_Pages 65-76.pdf

PDF . 616.9kB

Link

https://ejpb.journals.ekb.eg/article_256166.html

Detail API

https://ejpb.journals.ekb.eg/service?article_code=256166

Order

256,166

Type

Original research

Type Code

1,909

Publication Type

Journal

Publication Title

Egyptian Journal of Plant Breeding

Publication Link

https://ejpb.journals.ekb.eg/

MainTitle

CALLOGENESIS AND PLANT REGENERATION VIA IN VITRO CULTURE OF STEVIA REBAUDIANA EXPLANTS

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Type

Article

Created At

23 Jan 2023