The present study included 38 long staple genotypes, and two isolated hybrids in addition to two check varieties, Giza 90 and Giza 95. They were evaluated in 2019 season at Beni-sueif government (Trail A). While, Trail B consisted of 17 genotypes, selected from Trail A, with the three control genotypes, and evaluated in 2020 season at five locations (Beni-sueif, El-Fayoum, Assiut, Souhag and Luxor Governorates). Results of trail A indicated that, the two superior genotypes ([(G 83 × (G 75 × 5844)) × G90] × G91) and ({(G 83 x G 80) × Dandara)} × (G90 × A105)) were significantly out-yielded the two check varieties in seed, lint cotton yields and boll weight. Results of trail B showed that, locations significantly differed for all the studied traits, The genotypes and interactions mean squares were significant for seed cotton yield, lint cotton yield and boll weight. Mean performance across all locations showed that, ([(G 83 × (G 75 × 5844)) × G90] × G91), ({G 83 × (G 72 × Dandara)} x S109) and (G90 x A105) x G85 significantly out yielded the best check variety Giza-95 in seed cotton yield across all locations. Boll weight data indicated that, ((G91 × G90) × R101) and ((G91 × G90) × G80) had bolls heavier than the three checks. Results of trails A and B indicated that, the promising genotype ([(G 83 × (G 75 × 5844)) × G90] × G91) was significantly higher yield than the check genotypes in both seed and lint cotton yield in both trail A and trail B across all locations, and this cross may be considered as a promising material for future breeding programs to develop and isolate high yielding varieties of Egyptian cotton.