Two field experiments were carried out during 2019 summer season at two diverse locations of Gemmeiza and Mallawy Experimental Stations of Agricultural Research Center (ARC), Egypt, to evaluate twenty-six new yellow three-way crosses resulted from mating of 13 yellow maize inbred lines with two yellow single crosses, i.e. S.C 162 and S.C 168, as testers, using line × tester mating design. Also, to compare performance of these new three-way crosses with the best commercial three-way cross TWC 368. Results showed that location mean squares were significant or highly significant for all studied traits. Mean squares due to crosses were highly significant for all the studied traits. Mean squares due to lines and testers and their interaction were significant or highly significant for all studied traits, indicating that non-additive gene effects were more important than additive ones in the inheritance of all studied traits, except for days to 50 % silking. Two crosses (L1 × SC 162 and L1 × SC168) surpassed significantly in grain yield/fed the commercial hybrid TWC 368. The best in general combining ability effects were; inbred lines L7, L9, L11 and the tester SC168 for earliness, L4, L5, L7, L11and SC 168 for plant height, L4, L6, L11, L12 and SC 168 for ear height and L1 and SC 162 for grain yield. The best specific three-way combinations were; L3 × SC 162 and L6 × SC 168 for earliness, L9 × SC 168, L10 × SC 168 and L11 × SC 162 for plant shortness, L9 × SC 168 for lower ear position and L8 × SC 168 for grain yield.