This study aimed to monitor the incidence of Pseudomonas aeruginosa among patients in different intensive care units at Luxor International Hospital (LIH), one of the largest hospitals in Upper Egypt. Of the ninety-six isolates of Pseudomonas aeruginosa collected in 2019, from intensive care units, seventy-five of them were multidrug-resistant bacteria (MDR). About 30% of these isolates were collected from burn patients. Pseudomonas aeruginosa has in recent years been considered a hospital-acquired infection. Isolates were identified by biochemical assays and confirmed by Vitek2 compact system. Antimicrobial susceptibility testing of all ninety-six P. aeruginosa isolates were performed using the Kirby-Bauer disc diffusion method using thirteen different antibiotics and confirmed by Vitek2. All strains of P. aeruginosa showed different resistance to all groups of antibiotics ranging from 80% to 100 % such as Cephalosporins and Carbapenems, and ranged from 66.7% to 74.4% in some groups of antibiotics such as Aminoglycosides and Quinolones. The study indicated the high sensitivity of P. aeruginosa to the group of polypeptides of antibiotics by antibiotic Colistin, which was tested by the Minimum Inhibitory Concentration (MIC) method using the Vitek2 compact system.