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205265

Safe disposal of <i>E. coli</i> DH5α strain from liquid broth and solid substrate.

Article

Last updated: 05 Jan 2025

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Subjects

-

Tags

Bioremediation
Ecology
Microbiology

Abstract

Healthcare waste contains potentially infectious agents that represent risks for human health and the environment. Many techniques can be used to decontaminate these infectious wastes and make the management process safer and less costly. In the present study, various chemical disinfectants were used to decontaminate concentrated E. coli DH5α strain bacterial broth used in laboratories, as well as gauze contaminated with these bacteria. Dose-response statistical regressions were performed after multiple comparisons using the Kruskal-Wallis test with Nemenyi post-hoc. Fifty percent lethal concentration (LC50) for E. coli DH5α was 0.0586% for sodium hypochlorite (NaOCl), 0.0243 % for hydrogen peroxide (H2O2), 0.0161 % for formaldehyde, 12.20 % for ethanol, and 0.00401% for quaternary ammonium (BADAC-DDAC). When used to treat solid waste, all the chemicals used were able to completely disinfect the gauze when the concentration of 5×LC50 was used. This research highlights the adoption of biocides as efficient, low-cost alternative healthcare waste treatment options.

DOI

10.21608/jesj.2020.205265

Keywords

Infectious waste, Healthcare waste, Chemical decontamination, Waste treatment

Authors

First Name

Gustavo

Last Name

Peixoto

MiddleName

V. M.

Affiliation

Research and Development Division, Ezequiel Dias Foundation, Belo Horizonte, Minas Gerais, Brazil.

Email

-

City

-

Orcid

-

First Name

Marcos

Last Name

Mol

MiddleName

P. G.

Affiliation

Research and Development Division, Ezequiel Dias Foundation, Belo Horizonte, Minas Gerais, Brazil.

Email

-

City

-

Orcid

-

First Name

Pedro

Last Name

Castro

MiddleName

H. C.

Affiliation

Research and Development Division, Ezequiel Dias Foundation, Belo Horizonte, Minas Gerais, Brazil.

Email

-

City

-

Orcid

-

First Name

Maria Eduarda Machado

Last Name

Martins

MiddleName

E. M.

Affiliation

Research and Development Division, Ezequiel Dias Foundation, Belo Horizonte, Minas Gerais, Brazil.

Email

-

City

-

Orcid

-

First Name

Bruno

Last Name

Aleixo

MiddleName

S.

Affiliation

Research and Development Division, Ezequiel Dias Foundation, Belo Horizonte, Minas Gerais, Brazil.

Email

-

City

-

Orcid

-

First Name

Alessandra

Last Name

Matavel

MiddleName

-

Affiliation

Research and Development Division, Ezequiel Dias Foundation, Belo Horizonte, Minas Gerais, Brazil.

Email

alessandra.matavel@funed.mg.gov.br

City

Minas Gerais

Orcid

-

Volume

23

Article Issue

1

Related Issue

28934

Issue Date

2020-12-01

Receive Date

2021-11-17

Publish Date

2020-12-01

Page Start

1

Page End

12

Print ISSN

2314-5579

Online ISSN

2682-3942

Link

https://jesj.journals.ekb.eg/article_205265.html

Detail API

https://jesj.journals.ekb.eg/service?article_code=205265

Order

1

Type

High quality original papers

Type Code

1,869

Publication Type

Journal

Publication Title

Journal of Environmental Studies

Publication Link

https://jesj.journals.ekb.eg/

MainTitle

Safe disposal of <i>E. coli</i> DH5α strain from liquid broth and solid substrate.

Details

Type

Article

Created At

23 Jan 2023