Reactive oxygen species (ROS) is collective term both oxygen radical, such superoxide (O₂•), hydroxyl (OH•), peroxyal (RO₂•), and hydroperoxyl (HO₂•), and certain non-radical oxidizing agents, such as hydrogen peroxide (H₂O₂), hypochlorous acid (HOCL), and ozone (O₃), that can be convert easily to radical. The importance of antioxidants is shown here punicalagin. Punicalagin prevents harmful effect of (ROS) in all cell especially gonadal cells. So, the aim of study was to investigate effects of punicalagin (PL) on maternal live body weight (MLBW) during pregnancy in virgin rabbit does. In normal state, the decline in levels of free radicals is coupled with increased antioxidant and the reverse is true, but there is a critical balance between them during the development. Also, redox signaling induced by environmental pollutants (stressors) involve both alterations in antioxidant defenses and accumulation of ROS leading to oxidativestress which acts as a critical pathophysiological mechanism. This disturbance has deleterious effect on female reproductive.ROS due to environmental pollutants may result with high risk of any type of diseases producing developmental delay. Thus, maintaining the balance between antioxidants and ROS during pregnancy or lactation period may play an important role in maternal live body weight.