In this study, two novel marine fungal isolates (Aspergillus oryzae and Fsarium solani) were used for generating silver nanoparticles (SNPs). The antibacterial effect of mycological synthesized SNPs (12.31±0.26 and 22.6±1.2nm for A. oryzae and F. solani, respectively) was tested against Gram-positive (Bacillus cereus SBTBC, Enterococcus faecalis 8J, Lesteria monocytogenes 10403S and Staphylococcus aureus 7A) and Gram-negative bacteria (E. coli and Salmonilla sp.). Silver NPs exhibited higher bactericidal effect against Gram-negative strains compared to the Gram-positive ones. The maximal inhibitory concentration for Bacillus cereus SBTBC, Enterococcus faecalis 8J, Escherichia coli and Salmonilla sp. was 1.97, 1.64, 0.26 and 0.25 mg/mL, respectively, for synthesized SNPs by A. oryzae filtrate. Whilst it was, 9.31, 6.12, 2.61 and 0.921 mg/mL respectively, for synthesized SNPs by F. solani filtrate against the same bacterial strains. Our results show that mycological synthesized SNPs are promising compounds as antibacterial agents, however the exact mechanism of their bactericidal effect needs to be elucidated, and the safety of using them commercially still need to be more confirmed.