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204048

Screening and optimization of extracellular cellulase and pectinase enzymes produced from post-harvest fungi of apple (Pyrus malus L.) and tomato (Solanum lycopersicum L.)

Article

Last updated: 23 Jan 2023

Subjects

-

Tags

Microbiology

Abstract

Fourty-eight species and two species varieties belonging to 19 genera were collected from 50 samples of apple and tomato fruits (25 from each) from Sohag governorate in Egypt on dichloran rose-Benegal chloramphenicol agar medium at 28ᴼC. The most common genera were Alternaria, Aspergillus, Cladosporium, Fusarium and Penicillium. Form the above genera the most prevalent species were Alternaria alternata, A. tenuissima, Aspergillus niger, A. flavus, A. fumigatus, Cladosporium cladosporioides, C. herbarum and Penicillium oxalicum. Fourty-eight species and two species varieties fungal species screened for their abilities to produce cellulase (C1 enzyme) and pectinase enzymes. Six and seven species showed high cellulolytic and pectinolytic activity, while 23 and 17 species were moderately activity for the two enzymes, respectively. The remaining species were low activity in both enzymes. The highest cellulase and pectinase activities were recorded by Aspergillus chevalieri. Maximum production of cellulase enzyme by A.chevalieri was obtained after 6 days of incubation at 30ᴼC with initial pH 6 in culture medium containing sucrose and peptone as carbon and nitrogen sources, respectively. Regarding to pectinase enzyme the highest pectinase production by A. chevalieri was recorded after 6 days of incubation at 30ᴼC with initial pH 8 in culture medium containing pectin and ammonium sulphate as carbon and nitrogen sources, respectively.

DOI

10.21608/jesj.2017.204048

Keywords

mycology, Postharvest diseases, extracellular enzymes, Cellulase, Pectinase, Apple, Tomato, Aspergillus chevalieri

Authors

First Name

A.

Last Name

El-said

MiddleName

H.

Affiliation

Biology Department, Faculty of Science, Taif University, Kingdom of Saudi Arabia.

Email

-

City

-

Orcid

-

First Name

Hussein

Last Name

A.

MiddleName

M.

Affiliation

Biology Department, Faculty of Science, Taif University, Kingdom of Saudi Arabia. , Botany Department, Faculty of Science, South Valley University, 83523 Qena, Egypt

Email

-

City

-

Orcid

-

First Name

Thanaa

Last Name

Maghraby

MiddleName

A.

Affiliation

Botany Department, Faculty of Science, South Valley University, 83523 Qena, Egypt

Email

-

City

-

Orcid

-

First Name

Safa

Last Name

Meghezel

MiddleName

M.

Affiliation

Botany and Microbiology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt.

Email

safa_meghezel@yahoo.com

City

Sohag

Orcid

-

Volume

16

Article Issue

1

Related Issue

28205

Issue Date

2017-03-01

Receive Date

2016-05-14

Publish Date

2017-03-01

Page Start

21

Page End

36

Print ISSN

2314-5579

Online ISSN

2682-3942

Link

https://jesj.journals.ekb.eg/article_204048.html

Detail API

https://jesj.journals.ekb.eg/service?article_code=204048

Order

3

Type

High quality original papers

Type Code

1,869

Publication Type

Journal

Publication Title

Journal of Environmental Studies

Publication Link

https://jesj.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

23 Jan 2023