The aim of the present study was to separation and investigation of many amino acids (glycine, aspargen, leucine, glutamic acid, proline, argenine, valine, alanine) from the leaves and seeds of leucaena glauca L. Amino acids were separated and investigated by highperformance liquid chromatography (HPLC) technique, which consisted of a UV-vis detector, C18, Mobile phase was Na2SO4. The result showed that the sample of chloroform extract of leaves was recorded of the highest quantity of aspargine (43.725%), leucine (8.905%) glutamic acid (4.300%), while leucine, proline, alanine were recorded the highest quantity in seeds (29.575%, 19.031%, 14.115%) respectively.