The influence of root endophyte fungus Piriformospora indica on some crop plants was studied under pot conditions. The investigated plants were Vicia faba, Lupinus termis, Arachis hypogaea and Hibiscus sabdariffa. The presence of root endophyte fungus Piriformospora indica resulted significant increasing in plant biomass i.e. fresh, dry weight and length for both root and shoot compared with the non-colonized plants for all tested plants. Photosynthetic pigments content in leaves plant tissue appeared significant increasing in colonized plants compared to non-colonized plants in all tested plants, increasing ratio of total photosynthetic pigments were 21%, 50%, 16% and 13% in cases V. faba, L. termis and A. hypogaea, H. sabdariffa, respectively. The soluble proteins of various tested plants exhibited higher contents in colonized plants by P. indica than those of non-colonized plants. Moreover, enhance water relations where transpiration rate showed a significant increase in colonized plants by P. indica. Enhancement percentages were 14%, 64%, 121% and 92% in V. faba, L. termis, A. hypogaea and H. sabdariffa, respectively. Sodium content in colonized plants appeared decreasing content compered to non-colonized plants. Decreasing ratios were 16%, 10%, 13% and 20% in V. faba, L. termis, A. hypogaea and H. sabdariffa, respectively. Furthermore, calcium content in colonized plants was better than calcium content in non- colonized plants. Where, increasing ratios were, 25%, 40%, 30% and14% in V. faba, L. termis, A. hypogaea and H. sabdariffa, respectively. Finally, potassium content appeared significant increasing in colonized plants 25%, 16%, 40% and 45% in V. faba, L. termis, A. hypogaea and H. sabdariffa, respectively compared to non-colonized plants.