The influence of exogenous application of herbicides and salicylic acid (SA) on total amino acids and proline contents of two peanut cultivars was studied. Fusilade and basagran treatments caused an increase in the contents of free amino acids with 0.5 field dose (0.5 FD) and 1 FD but decreased with 1.5 FD. SA affects greatly the leaf amino acids contents with or without herbicides. Spraying of SA one day before fusilade treatment caused a significant increase in amino acids contents of Giza 5 (G5) and Giza 6 (G6) comparing with that of the corresponding controls. SA treatments, mixed with or sprayed before basagran application, caused no significant changes in leaf amino acids content compared to the control. G6 cultivar contained almost doubled amount of proline compared to that of G5. Spraying of SA induced proline content in G5 and G6 leaves. G5 showed a high accumulation of proline but G6 leaves contained low amounts of proline due to SA + fusillade treatments. Leaf proline content of G5 increased with increasing basagran doses. Treatment of G6 cultivar with basagran showed no significant change of leaf proline content in comparison with that of the control. Application of SA to the highest dose of basagran enhanced more accumulation in proline content of G6 leaves.