Worldwide, foodborne diseases are most commonly caused by Salmonella. However, chicken giblets and washing water are the most frequent and good indicators of bacterial food poisoning. Additionally, the paucity of information regarding Salmonella microbial resistance in developing nations. In this investigation, fifty-five chicken livers and washing water samples were gathered from the small-scale poultry processing plants in Cairo and Giza, Egypt. A Salmonella occurrence was detected, identified, and finally phenotypically characterized for the most common antibiotic groups to detect its antibiotic resistance profiles. Salmonella species have been recovered from 1 out of 45 (2.2%) examined chicken livers. This isolate was biochemically identified and molecularly verified such as Salmonella by the invA molecular marker target gene detection using PCR. The Salmonella serovar was recognized as S. Anatum. In our study, S. Anatum showed resistance to ten antimicrobial agents among six antimicrobial classes, so this isolate was classified as MDR. S. Anatum showed resistance to gentamicin, ampicillin, ciprofloxacin, tetracycline, chloramphenicol, cefepime, ceftazidime, ceftriaxone, cefotaxime, and aztreonam. In conclusion, chicken livers were contaminated with MDR Salmonella serovars, which could be extremely dangerous for human health. To control such food poisoning hazards, the necessity to implement food safety systems is imperative. Additionally, continuous updating of the occurrence and antibiotic resistance profile regarding Salmonella is an important food safety issue.