This work monitored the aflatoxin M1 (AFM1) in raw buffalo milk trailed in Sohag city, Egypt in different seasons. Milk samples were obtained from March to October 2021. The enzyme-linked immunosorbent assay(ELISA) was used as a methodology technique. The results of AFM1 presented that the highest frequency of occurrence, with a detection incidence of 85.5 % in winter samples and 78.5% in summer samples. The positive samples showed range levels of AFM1 between 0.06 to 0.8μg/kg in winter samples and range of 0.0 to 0.9 μg/kg in summer milk samples. The percentage of aflatoxin M1 samples exceeded the MRL of Egyptian regulation (ER) (2010) 2010/7136 last updated, are 78 and 100% in two examined seasons, respectively.The residue levels of AFM1 obtained in the investigated samples; represented a serious concern about the health risk of consumers. It is worthy to sit a regular schedule for monitoring and inspection of dairy products for aflatoxin residues.