Qualitative and quantitative determination of the amino acid pat tern in buffaloe's milk have been carried out in 12 bluk milk samples collected from lactating buffalo-cow's Seventeen amino acids have been identified and compared with the amino acid contnet of egg protein as well as cow's milk protein. Buffalo's milk protein was found rich in the essential amino acids, methionine, phenylalanine and valine compared to cow's milk protein. Compared with egg protein, the animo acids, valine, phenylalanine and methinonine showed higher values. whereas thireonine, arginine aspartic acid, cystine, glycine and serne were found lower. Buffalo-cow's milk was found particularly rich in valine, which recommend its use as a supplement to a valine difficien diets.