The effect of some stress factors on blood corticoids, leucoctyic count, and glucose in chickens has been investigated.
1.--Blood corticoids in chickens of 52 and 58 days of age have been determined by using two different methods depending on com petitive protein binding assay (CPB).
2.-Comparison between these two methods CPB method using SH--B corticosterone and the Cortipac CPB assay using Selenium labelled Cortisol, proved that the last method can be satisfactorily used
3.-Injection of ACTH significantly increased the level od cortic oids, eosinophils and pseudoeosinophils. Variations in total leucocy tic and blood glucose level were not significant.
4.-Under heat stress (40°C) significant increase of blood corticoids and significant decrease in the blood glucose, eosinophils , and pseud oesinophils was evident. Death occured after two hours.
5.-Deprivation of food and water caused significant increase in the blood corticoids after 12 hours. Other variations in the blood parameters were not significant.
6.-Vigorous exercise significantly increased blood corticoids after one hour and blood sugar after two hours. Leucocytes showed no significant change.