Blood serum samples (205) were collected from clini cally healthy cattle and buffaloes to establish the normal pancreatic function with regard to species, sex and age. Species differences were not detected in lipase, amylase and trypsin enzyenes levels. Sex pla yed a minor role while age had a significant effect. Sex had no effect upon liver enzymes (S-GOT; S-GPT, alkaline and acid phosphatase) while they were in creased by increasing age. Comperativelys buffaloes had a higher level of S-GOT and S-GPT, of total lipids and glucose than cattle. On the other hand cattle had higher levs of triglycerides and total cholesterol. Sex seemed to exert neglicable or even no role on to tal lipids, cholesterol triglycerides and glucose. Aged animals had a higher levels of total lipids, to tal cholesterol, triglycerides and glucose while age seemed to play no role an total protein.