Effect of salting (10% and 20%) and that of freezing on the metacercariae of Haplorchis pumilio and Prohe mis tomum vivax infecting the fresh water fish Alestes nurse in Assiut Province is studied. Test of the viability of the metacercariae was done not only by microscopic examination but also by experimental in fection in albino rats. Effect of freezing was also studied on the same matacercariae and was found to be more drasting than the effect of salting. Both meta cercariae were found to be less resistant than the metacercariae of Heterophyes heterophyes which is more commonly encountered as a human parasite of Lower Egypt.