The serum enzymatic activity (S-GOT, S-CPT and S-AP) and the haematological changes (RBCs, PCV, WBCs and differential leucocytic count) of 30 Buffaloes were determined from the last 4 weeks of pregnancy to the 6th week after parturition. Further 17 non lactating buffaloes were considered as a control group. The enzymatic activity were found to increase with the advance of pregnancy especially during the last week, while the haematological changes showed decreasing values. After parturition the enzymatic activity decreased while the haematological values increased till they approach the values of the control group after 6 weeks post parturition.