Histological details of the eyelids from 20 adult Egyptian buffaloes were observed. The epidermis is relatively thin in both the superior and inferior pelids, but becomes thicker and heavily pigmented on the free margin between the cilia and the tarsal gland openings. The palpebral surface is lined with transitional epithelium containing goblet cells. Morphologically, four types of sebaceous glands could be recognized. Dermoepidermal free nerve endings, free nerve endings surround the hair follicles and krause's end bulbs were demonstrated. The distribution and activities of alkaline phosphatase, acid phosphatase and succinic dehydrogenase were investigated.