The distribution of free protoplasmic droplets and spermatozoa with proximal and distal ones was studied in the different regions of 16 reproductive tracts as well as in the first and second ejaculates of 3 buffalo bulls. The percentage of free protoplasmic droplets was very low in the anterior third of the caput (4.83+1.10%) and it increa sed gradually in successive parts of reproductive tract till reached its maximal level (59.08+4.06%) in the ampulla. Spermatozoa with proximal droplets were prevalent in the anterior third of the caput, while spermatozoa with distal ones were prevalent in posterior third of cauput, body, tail and vas deferens.
The percentage of free protoplasmic droplets in the first ejaculate was 54.42+2.76% while in the second ejaculate it was significantly increased to 67.44+3.03%. A significant increas in the percentage of spermatozoa with distal droplets was also recorded in the second ejaculate.