The most suitable sites for injection and arthrocentesis of different sacs of the tarsal joint in donkey were exactly determined. The anatomical features were investigated. They differ from those in the horse in that the proximal surface of the fused first and second tarsal bone has only one facet for articulation with the central tarsal bone. The facet on the proximal surface of the central for articulation with the calcaneus which is some times present in horse, is not recorded in the present work. moreover, the donkey has an additional short lateral collateral ligament superfical to the long one. A slit like opening is found to be the site of communication between the tarsocrural and proximal intertarsal sac.