One hundred and thirty swiss-albino mice of 3-4 weeks old were each inoculated intraperitoneally (1/p) with a fixed dose of RVE virus.
The first symptoms started to appear after 42 hours. The course of the disease progressed and the symptoms became apparent between 54 to 102 hors, then started to decline by 114 hours. Mice surviving at the end of the experiment did not manifest any clinical symptoms.
Livers, spleens, brains, kidneys, hearts, lungs, intestines and skeletal muscles were collected at predetermined intervals for quantitative assay of the virus content in the verious organs. Virus content as detected by titration in CER cell cultrues was higher in the liver than the heart, spleen, lung, kidney and less in the brain. Yet, virus could not be isolated from the intestines and skeletal muscles of infected mice.