26 (25.24%) isolates of Pseudomonas spp, were detected from 103 dead chicken-embryos. In addition 18.23%, 6.35%, 5.55% and 1.54 of Pseudomonas spp < /strong>, were recovered from baby chicks, growing chickens, laying hens and apparently healthy birds respectively. The isolated Pseudomonas spp (68 strains), were bicohemically differentiated into 5 species including: Ps. aeruginosa (5.58%); Ps. vesicularis (4.96%); Ps. aureofaciens (1.24%); Ps. diminuta (1.24%) and Ps. flava (1.03%).
Pathogenicity tests in 7-day old chicken embryos by yolk-sac in oculation or dipping in broth culture of Ps. aeruginosa (Reference strain), Ps. aeruginosa (clinical isolate) and Ps. vesicularis clinical isolated proved that these organisms were highly pathogenic with high mortality rate. Similar results were obtained by using the filtrates of the previous organisms by yolk sac inoculation. On the other hand results of less p < /strong>athogenicity with low mortality were recorded by using the filtrates of the previous organisms through dipping.
The experimental infection in baby chicks by different routs with the previous cultures or their filtrates, revealed that the subcutaneous route of inoculation was highly effective.
The isolated Pseudomonas spp from dead embryos and baby chicks were sensitive in vitro to Rifampin, Neomycin and garamycin.