Bacteriological examination was performed on fifty fresh Nile fishes. A total of 212 bacterial strains were isolated from muscles, livers and gastrointestinal tracts of some common fishes namely Tilapia nilotica, Clarias lazera, Bagrus bayad, Synodontis schall and Schilbe mystus in Assiut City. All the examined samples were accepted organoleptically. Strept faecalis, Strept faecium, Staph aureus, Staph epidermidis, Micro cocci, E. coli, Klebsiella aerogeness, Enterobacter aerogenes, Citrobacter freundi, Shigella dysenteriae, Shigella flexneri 6, Proteus rettegeri, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Providencia, Edwardsiella tarda; Serratia marscens, Alcaligenes faecalis and Achromo bacter were isolated in different percentages. No Salmonella species
could be recovered in the present investigation. Four strains of entero pathogenic E. coli: 0 55, 0 86 a, 0119 and 0 128 a 0 128 b could be identified. Tilapia nilotica was found to harbour large number of bacterial sp < /strong>ecies more than the other types of fish examined. On the other hand, the gastro-intestinal tracts of the fish examined were found to contain large numbers of microorganisms. Correlation between the bacterial isolates and kinds of fish as well as the public health significance of the isolated organisms were discussed.